Preparation of DNA Containing 5‐Hydroxymethyl‐2′‐Deoxycytidine Modification Through Phosphoramidites with TBDMS as 5‐Hydroxymethyl Protecting Group
نویسندگان
چکیده
منابع مشابه
Deprotection of t-Butyldimethylsiloxy (TBDMS) Protecting Group with Catalytic Copper (II) Chloride Dihydrate
The protection of functional group is unavoidable in multi-step organic synthesis. Along with tetrahydropyranyl (THP) ethers, t-butyldimethylsilyl (TBDMS) ethers have been widely used for protecting hydroxyl groups. TBDMS ether is more stable to hydrolysis than trimethylsilyl ether, but is still readily cleaved by a variety of selective conditions. The deprotection of TBDMS is usually under mil...
متن کاملA Direct Method for β-Selective Glycosylation with an N-Acetylglucosamine Donor Armed by a 4-O-TBDMS Protecting Group.
A new direct method for β-selective glycosylation with an N-acetylglucosamine (GlcNAc) donor was developed. This substrate, which can be readily prepared from commercially available GlcNAc in two steps, contains a 4-O-tert-butyldimethylsilyl (TBDMS) protecting group as a key component. We found that this functionality could have a favorable effect on the reactivity of the GlcNAc donor. Glycosyl...
متن کاملPreparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries
The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library. The challenge in gene library preparation is to achieve controlled total or partial randomization at any predefined number and position of codons of a given gene, in order to obtain a library with a maxim...
متن کاملsynthesis and dna interaction studies of new cobalt (ii) complexes containing different dinitrogen aaromatic ligands with calf thymus dna using different spectroscopy methods
در این مطالعه بر هم کنش این چهار کمپلکس با dna تیموس گوساله توسط روش های اسپکتروفتومتری، فلوریمتری، دو رنگ نمائی دورانی، و ویسکوزیمتری بررسی شدند تا اثر استخلاف های متیل بر روی لیگاندهای فنانترولین بررسی گردد.
Sulfurizing Reagent Ii and Its Use in Synthesizing Oligonucleotide Phosphorothioates
Preparation of oligoribonucleotides with a phosphorothioate backbone requires a sulfurizing reagent that is efficient and stable in solution. One of the most efficient sulfurizing reagents, 3H1,2-benzodithiol-3-one-1,1-dioxide (Beaucage Reagent), is stable in solution but lacks longterm stability on the DNA synthesizer. This report evaluates Beaucage Reagent and 3((N,N-dimethyl-aminomethylidene...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Nucleic Acid Chemistry
سال: 2011
ISSN: 1934-9270,1934-9289
DOI: 10.1002/0471142700.nc0447s47